from twisted.internet import reactor, defer
from scrapy.crawler import CrawlerRunner
from scrapy.utils.log import configure_logging

class MySpider1(scrapy.Spider):
    # Your first spider definition
    ...

class MySpider2(scrapy.Spider):
    # Your second spider definition
    ...

configure_logging()
runner = CrawlerRunner()

@defer.inlineCallbacks
def crawl():
    yield runner.crawl(MySpider1)
    yield runner.crawl(MySpider2)
    reactor.stop()

crawl()
reactor.run() # the script will block here until the last crawl call is finished